Visualizing liquid-liquid phase separation and protein aggregates.

ABSTRACT: Liquid-liquid phase separation (LLPS) facilitates functionally-relevant proteins to gather in high concentrations at specific locations. Several interacting proteins encapsulated in the membraneless droplets tend to form abnormal protein aggregates which are linked to numerous pathological diseases. Thus direct observation of LLPS, successive protein aggregates and the potential conversion processes is significant for understanding associated cell function and pathology. In this review, we firstly introduce the techniques for imaging LLPS, including in vitro reconstitution and intracellular visualizing strategies. Then we elaborate recent advances in the development of the imaging approaches for protein aggregates, followed by highlighting the monitor of protein conformational changes duri

SUBMITTER: Xue J 

PROVIDER: S-EPMC12647606 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature