Development of mumps virus-specific sialidase imaging probes through chemical modifications of sialic acid.

ABSTRACT: Some viruses, such as mumps virus (MuV), possess sialidases that cleave terminal sialic acids, primarily N-acetylneuraminic acid (Neu5Ac), from glycans. Previously, we developed a sialidase fluorescent imaging probe (BTP3-Neu5Ac) and visualized cells infected with sialidase-expressing viruses. However, BTP3-Neu5Ac could not specifically identify these viruses, as it reacted with a wide range of sialidases from viruses, bacteria, and mammals. Here, to confer specificity of BTP3-Neu5Ac to MuV sialidase, which features a hydrophobic cavity extending toward the C5 position of bound Neu5Ac, we evaluated BTP3-Neu5Ac derivatives containing hydrophobic groups at the C5 position of Neu5Ac moiety across various viral sialidases, including influenza A and B viruses. Linear N-acyl groups with 5-8 carb

SUBMITTER: Narimichi Y 

PROVIDER: S-EPMC12647717 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature