Single-cell and spatial transcriptomics implicate a prognostic function of tertiary lymphoid structures in gastric cancer.

ABSTRACT: Tertiary lymphoid structures (TLSs) have emerged as critical prognostic and immunotherapeutic indicators in cancer, with their clinical significance modulated by spatial distribution patterns and density. Here, we performed integrated single-cell and spatial transcriptomic profiling of 30 gastric cancer (GC) specimens stratified by TLS spatial localization patterns. Comparative analysis shows pronounced enrichment of CXCL13⁺ T lymphocytes (TLCs), CXCR5⁺ germinal center B lymphocytes (gc_B cells), LAMP3⁺CD80⁺ activated dendritic cells (DCs), and SELP⁺ACKR1⁺ high endothelial venule (HEV) cells within intratumoral-TLS (iTLS) rich tumors compared to peritumoral-TLS (pTLS) and desert-TLS (dTLS) tumor subtypes. Multimodal cell-cel

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12647882 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature