MtDNA-depleted neuronal cell transcriptomes reveal Alzheimer's disease-related changes.

ABSTRACT:

Introduction

We determined whether mitochondrial DNA (mtDNA) depletion induced Alzheimer's disease (AD)-relevant transcription changes.

Methods

Following RNA sequencing (RNA-seq), we identified differentially expressed genes (DEGs) between SH-SY5Y or NT2 mtDNA-depleted (ρ0) and intact (ρ+) cell lines and quantified concordant DEG changes. Gene set enrichment analysis and over-representation analysis were used to determine the impact on the Kyoto Encyclopedia of Genes and Genomes (KEGG) AD and other neurodegenerative disease pathways, ascertain pathway and term enrichment in the Reactome and Gene Ontology databases, and generate Ingenuity Pathway Analysis z-scores.

Results

Relative to their ρ+ comparators, ρ0 lines differentially expressed >75% of their genes. The KEGG

SUBMITTER: Menta BW 

PROVIDER: S-EPMC12647984 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature