MTA1 upregulation enhances stemness and chemoresistance of gastric cancer cells.

ABSTRACT:

Objective

MTA1 (Metastasis-associated protein 1) is implicated in various malignancies, but its role in gastric cancer (GC) stemness and chemoresistance remains unclear. This study explores the effect of MTA1 on these processes and its underlying mechanisms.

Methods

MTA1 expression was assessed in GC tissues, paracancerous tissues, and cell lines. Lentiviral vectors were used to overexpress or silence MTA1 in AGS cells. Functional assays included MTT for drug sensitivity, flow cytometry for apoptosis, sphere formation to assess stemness, and Western blot for SOX-2, OCT-4, Bax, and Bcl2 expression. In addition, TCGA gastric cancer datasets were analyzed to evaluate the association of MTA1 expression with patient prognosis and predicted cisplatin sensitivity.

Results

MT

SUBMITTER: Zhou H 

PROVIDER: S-EPMC12648086 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature