Single-Stranded RNA Origami Remodels Macrophage to Promote the Synergistic Antitumor Effect with 5‑Fluorouracil in Pancreatic Cancer.

ABSTRACT: The advancement of cancer immunotherapy has focused on developing therapies that not only target tumor progression but also enhance immune responses, which could potentially shift the balance within the tumor microenvironment (TME) to promote a more immune-competent environment and improve the effectiveness of antitumor immunotherapies. This study evaluates the internalization of single-stranded RNA origami (ssRNAOG) in coculture models mimicking the TME in vitro and the antitumor efficacy of the combination of ssRNAOG with 5-fluorouracil (5-FU) in pancreatic ductal adenocarcinoma (PDAC) models. The internalization of ssRNAOG triggers the TLR3 signaling pathway, leading to robust innate immune activation. Notably, ssRNAOG induces the overexpression of MHC class I protein on macrophages, wh

SUBMITTER: Tu X 

PROVIDER: S-EPMC12648283 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature