Molecular Insights into the Engagement of High-Affinity Sialylated Ligands to Human CD22.

ABSTRACT: CD22 is a sialic acid-binding immunoglobulin-like lectin (Siglec) that maintains a baseline level of B cell inhibition. Its function and restricted expression in B cells make CD22 a validated target in therapies against dysregulated B cells, which cause cancer and autoimmune diseases. High-affinity sialic acid-based ligands capable of competing with natural ligands to bind CD22 represent a promising therapeutic opportunity. Here, we describe the design and synthesis of a sialoside library constructed by chemical modifications on carbon C2 of 9-N-biphenylcarboxamide Neu5Ac (^BPCNeu5Ac) or 9-N-m-phenoxybenzamide Neu5FAc (^MPBNeu5FAc) scaffold using a copper-(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reaction. Subsequent analysis of binding to human CD2

SUBMITTER: Ereno-Orbea J 

PROVIDER: S-EPMC12648315 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature