A Hybrid G‑Quadruplex Structure Formed by a Sequence from the MAX Gene.

ABSTRACT: Here, we report the NMR solution structure of the G-quadruplex (G4) folded by the 24-nt guanine-rich DNA sequence d-[AG₃AG₄A₂G₄TGA₂G₄A]. The sequence is originated from the first intron of the MAX gene, which encodes the transcription factor MAX, an obligate partner of the notorious oncoprotein MYC. The unimolecular G4 adopts a [3 + 1] hybrid topology containing all the three main loop types (propeller, diagonal, and lateral). Also, the three G-tetrads are stacked with the same polarity of the Hoogsteen hydrogen bond and anti-anti-anti-syn glycosidic configuration pattern. Both 5' and 3'-flanking regions contain one adenine residue. As far as we know, it is the first example of such a unique topol

SUBMITTER: Hu X 

PROVIDER: S-EPMC12648320 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature