Enzymatic Synthesis and Anticoagulant Evaluation of Fucosylated Chondroitin Sulfate Polysaccharides with Defined Sulfation Patterns.

ABSTRACT: Fucosylated chondroitin sulfate (FCS), a uniquely structured glycosaminoglycan with outstanding anticoagulant activity and a lower risk of bleeding, is a promising anticoagulant candidate. However, the complex and heterogeneous structure of the natural FCS polysaccharide presents significant challenges in developing novel anticoagulant drugs and elucidating the structure-activity relationship. Herein, we developed an enzymatic system for the synthesis of FCS polysaccharides with defined sulfation and fucosylation patterns using bifunctional L-fucokinase-GDP-L-fucose pyrophosphorylase (BfFKP) and α1-3-fucosyltransferases mutant (Hpα1, 3-FucTM) and successfully synthesized FCS polysaccharides with α1-3-linked fucose (FCS_A), 4-O-sulfated fucose (F_4SCS_A

SUBMITTER: Li R 

PROVIDER: S-EPMC12648335 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature