Decoding protein N-phosphorylation functionality and sequence patterns.

ABSTRACT: Protein N-phosphorylation represents one of the principal forms of protein phosphorylation modifications, which has been extensively identified in both prokaryotes and eukaryotes. However, significant knowledge gaps persist regarding its functional implications, sequence motifs, and structural characteristics. Through integrated functional, sequential, and structural analyses of experimentally verified large-scale N-phosphorylation datasets, we found that protein N-phosphorylation broadly participates in diverse biological processes and exhibits close associations with human diseases, particularly cancer. And the high motif conservation between N- and O-phosphorylation in eukaryotes offers novel research perspectives for investigating N-phosphorylation through the lens of O-phosphorylation

SUBMITTER: Zhao MX 

PROVIDER: S-EPMC12648400 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature