Wide-Field Digital Surface-Enhanced Raman Scattering: Quantitative Single-Molecule Detection with High Sensitivity and Throughput.

ABSTRACT: Due to its exceptionally high sensitivity and specificity, surface-enhanced Raman scattering (SERS) is widely employed in diverse fields, including biomedicine, environmental monitoring, and food safety. Nonetheless, the lack of reproducibility and substrate uniformity has seriously hindered its application in quantitative detection, particularly at low analyte concentrations. Recently, the concept of digitization has been integrated into SERS, enabling quantitative and sensitive detection with promising applications (Bi et al. Nature2024, 628, 771-775). In this work, we further developed a wide-field digital SERS (WidiSERS) by employing wide-field microscopy for high throughput. Protein assembled gold nanorod dimers are used for largely enhancing the Raman signals. Reproducible quantifica

SUBMITTER: Ye S 

PROVIDER: S-EPMC12648421 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature