Inc/GFP chimera protein-based interactomics reveals host cellular interactions of Cps0558, a novel Chlamydia psittaci inclusion protein.

ABSTRACT: The obligate intracellular Gram-negative bacterium Chlamydia psittaci, a zoonotic pathogen transmissible between birds and humans, has played a pioneering role in research on its membrane-bound replicative niche termed the inclusion. Inclusion membrane proteins (Inc proteins) are crucial for Chlamydia-host interactions and were first identified in C. psittaci. This study investigates putative C. psittaci Inc proteins by a combination of in silico analyses, immunofluorescence and, strikingly, a new Inc/GFP chimera protein-based interactomics approach to identify host cellular interaction partners. Here, we report a novel C. psittaci Inc protein, Cps0558, along with respective host cellular interaction partners, in particular ACAD11, which is involved in lipid metabolism. We confirm their ph

SUBMITTER: Gensch JM 

PROVIDER: S-EPMC12648544 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature