<i>S. aureus</i> biofilm disruption using ultrasound and microbubbles: Influence of radiation force, bubble dynamics and biofilm growth conditions.

ABSTRACT: Staphylococcus aureus is a human pathogen and a major cause of bloodstream infections, which can readily form biofilms on implanted medical devices. Here, we utilise a combination of lipid-shelled microbubbles (MBs) and ultrasound (US) to physically disperse the biofilm from the growth surface. The effects of two peak negative pressures (PNPs) and the direction of the acoustic radiation force (ARF) were evaluated. At 1.1 MHz, a clinically relevant frequency, and low PNP of 360 kPa, no significant biofilm dispersal occurred regardless of ultrasound (US) orientation. In contrast, at a high PNP of 2500 kPa, directing the ultrasound beam upward (US↑) pushed microbubbles (MBs) toward the biofilm, resulting in near-complete dispersal of the biofilm (94 ± 2 %) within the focal zone. Revers

SUBMITTER: Batchelor DVB 

PROVIDER: S-EPMC12648606 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature