RNase P generated tRF^Ser-GCT promotes fat storage in adipocytes via Adrb2 signaling.

ABSTRACT: tRNA-derived small RNAs (tsRNAs) are emerging regulators of metabolism, but their roles in adipose tissue are not well defined. Here, we profiled tsRNA expression in mouse brown adipose tissue (BAT), gonadal white adipose tissues, and inguinal white adipose tissue (iWAT), revealing depot-specific patterns and a notable enrichment of mitochondrial tRF-5c fragments, especially tRF-1:29-chrM.Ser-GCT (tRF^Ser-GCT), in BAT. tRF^Ser-GCT expression correlated with mitochondrial abundance and increased during adipogenic differentiation and metabolic activation, both in vivo and in vitro. Functionally, tRF^Ser-GCT promoted adipogenesis and lipid accumulation in 3T3-L1 cells and localized to mitochondria. Mechanistically, tRF^Ser-GCT is generated from mitochon

SUBMITTER: Shen L 

PROVIDER: S-EPMC12648613 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature