Immobilization-free SELEX for aptamer discovery targeting colorectal cancer-derived small extracellular vesicles.

ABSTRACT:

Background

Despite the increasing prominence of small extracellular vesicles (sEVs) and liquid biopsies for early cancer diagnosis, the development of high-performance molecular probes specifically targeting sEVs remains limited. In this study, we present a novel enzymatic digestion sEV-systematic evolution of ligands by exponential enrichment (EDGE-SELEX) strategy that eliminates the need for sEV immobilization, thereby preserving the native structural and biochemical characteristics of sEVs and better mimicking their clinical environment.

Results

Using the EDGE-SELEX approach combined with a post-selection optimization process, we successfully identified two novel aptamers, H7F-3 and H15F, exhibiting high affinity for colorectal cancer (CRC)-derived sEVs, with dissociation

SUBMITTER: Lee ES 

PROVIDER: S-EPMC12648788 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature