Cell-free DNA methylation and fragmentomics-based liquid biopsy for accurate esophageal cancer detection.

ABSTRACT:

Background

Cell-free DNA is a promising source of biomarkers for early cancer detection and carries tumor-driven methylation and fragmentation features that have achieved good diagnostic efficacy across various cancers. However, there were no studies that detected both of them for esophageal cancer diagnosis.

Methods

In this study, we analyzed the cfDNA methylation and fragmentation markers for accurate esophageal cancer detection. Using cfMeDIP-seq, we profiled 145 plasma samples from healthy controls and esophageal cancer patients. We used multiple algorithms to identify cfDNA methylation markers and fragmentation markers to evaluate the efficacy of early esophageal cancer detection.

Results

Finally, we identified 25 cfDNA methylation and fragmentation markers and c

SUBMITTER: Wang J 

PROVIDER: S-EPMC12648842 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature