Characterization of a novel Jumbo phage JP4 with potential to control pathogenic Escherichia coli.

ABSTRACT:

Background

Amidst rising antimicrobial resistance, bacteriophage (phage) therapy has re-emerged as a pivotal weapon against multidrug-resistant pathogens. Jumbo phages, distinguished by large genomes, show particular therapeutic promise. Yet current understanding of jumbo phages is still lacking.

Methods

Phage was isolated from domestic sewage. The biological properties of JP4 was characterized via transmission electron microscopy, stability tests, one-step growth curve. The genome of JP4 were elucidated by sequencing and bioinformatics tools. Structural proteins were identified via mass spectrometry. Bactericidal and biofilm eradication activities were evaluated using bacterial turbidity measurements and crystal violet assays, respectively. Statistical significance was dete

SUBMITTER: Zhang K 

PROVIDER: S-EPMC12648861 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature