Mesenchymal stem/stromal cells overexpressing leukemia inhibitory factor (LIF) promote arteriogenesis and functional recovery in a mouse model of critical hindlimb ischemia.

ABSTRACT:

Background

Critical limb ischemia (CLI) is the most severe manifestation of peripheral arterial disease (PAD), for which there are still no effective therapeutic options. Mesenchymal stem/stromal cells (MSCs) can secrete bioactive molecules capable of promoting tissue regeneration and neovascularization. The therapeutic potential of MSCs can be enhanced by genetic modification to overexpress molecules of interest. Here, we produced an umbilical cord-derived MSC line overexpressing leukemia inhibitory factor (LIF), MSC_LIF, and evaluated its pro-angiogenic/arteriogenic potential.

Methods

MSCs were transduced using a second-generation lentiviral system to express LIF. Transgene overexpression was confirmed by qRT-PCR and ELISA. The transcriptional and secretory profile of MSC_

SUBMITTER: Santos GC 

PROVIDER: S-EPMC12649050 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature