A synthetic metabolic pathway for the de novo biosynthesis of medium chain length γ- and δ-lactones.

ABSTRACT:

Background

Medium chain length γ- and δ-lactones, in particular γ-decalactone, are potent aroma compounds used by the flavour and fragrance industry. The native pathways for their biosynthesis are unknown so to facilitate future biomanufacturing, we designed and implemented a novel metabolic pathway, using engineered enzymes, for the de novo biosynthesis of lactones, leading to the production of γ-decalactone, δ-decalactone, γ-dodecalactone and δ-dodecalactone in Escherichia coli.

Results

Wild-type enzymes with the appropriate substrate specificities for the pathway were not available, therefore enzyme engineering was required. Firstly, the Cuphea viscosissima FatB1 thioesterase was modified to be C10 specific, resulting in novel mutants with a 77.1% C10 specificity in E. co

SUBMITTER: Muller J 

PROVIDER: S-EPMC12649083 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature