Red LED Light Irradiation Increases the Resistance Against Environmental Stress of Frozen Bovine Sperm Thawed in Suboptimal Conditions.

ABSTRACT: This work sought to test the positive effect of red LED light on frozen bovine sperm resistance to thawing in suboptimal conditions. Moreover, a preliminary study explores whether this improvement could have any repercussions on in vivo fertilizing ability. Thus, frozen bull semen straws (n = 16) were thawed (a) with irradiation for 5 min at 20 °C (2 min light, 1 min darkness, 2 min light; PHOTO); (b) without irradiation for 5 min at 20 °C (ET); or (c) through immersion into a water bath at 38 °C for 40 s as standard control (CONTROL). Sperm quality and preliminary, purely descriptive AI trials were performed. The PHOTO samples demonstrated significantly (p < 0.05) improved percentages of intact acrosomes, progressive motility, DNA condensation and fragmentation, and viable sperm wi

SUBMITTER: Blanco-Prieto O 

PROVIDER: S-EPMC12649307 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature