Integrative Multi-Omics Analyses Reveal the Global Regulation Network of the Microalga <i>Nannochloropsis oceanica</i> Under Nitrogen Stress Adaptation.

ABSTRACT: Microalgae of the genus Nannochloropsis are known for their ability to accumulate large amounts of lipids, particularly triacylglycerides (TAGs), when exposed to nitrogen-limiting conditions. This trait makes them promising candidates for biofuel production. While previous studies have used transcriptomics and metabolomics to explore how these organisms respond to nutrient stress, the role of post-translational modifications-especially protein phosphorylation-remains poorly understood. To address this gap, we conducted a comprehensive analysis of protein phosphorylation events in Nannochloropsis oceanica under both nitrogen-replete and nitrogen-depleted conditions over a time-course experiment. Using mass spectrometry-based phosphoproteomics, we identified 1371 phosphorylatio

SUBMITTER: You W 

PROVIDER: S-EPMC12649847 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature