Directed Experimental Adaptive Evolution of Osmoregulation in Fungal Pathogen <i>Magnaporthe oryzae</i> Is Independent of Glycerol Metabolism-Associated Genes.

ABSTRACT: Directed experimental adaptive evolution in fungal pathogens is largely unexplored. In the phytopathogenic fungus Magnaporthe oryzae, long-term cultivation under osmotic stress was found to lead to individuals arising as suppressor strains out of osmosensitive "loss-of-function" mutants, in which the high osmolarity glycerol (HOG) pathway was inactivated. The underlying mechanisms of reestablished osmoregulation in the suppressor strains are not known. Here, we found that two different types emerged from the mycelium parts of each ∆Mohik1, ∆Moypd1, ∆Mossk1, ∆Mossk2, ∆Mopbs2, and ∆Mohog1: reversible suppressors, which still struggle with osmotic stress, and irreversible suppressors, which can cope with the same stress situations. This phenome

SUBMITTER: Bersching K 

PROVIDER: S-EPMC12650440 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature