Hypoxia Supports LPS-Driven Tolerance and Functional Activation in BV-2 Microglial Cells.

ABSTRACT:

Background

Prolonged hypoxia contributes to irreversible organ damage, particularly in the brain and heart. While chronic hypoxia is harmful, mild short-term hypoxia can trigger protective mechanisms. This study investigates how such hypoxic conditions affect BV-2 tolerant microglial cells in vitro, focusing on inflammation, metabolism, and functional activity. Although in vitro models provide a controlled setting, our findings may offer insights into microglial behavior in vivo under similar conditions.

Methods

We used various molecular and biochemical techniques to assess the inflammatory state of BV-2 microglia under hypoxia, measuring glycolytic activity (via lactate production), and evaluating migratory and phagocytic capacities in vitro.

Results

Hypoxic conditio

SUBMITTER: Chavero Vargas A 

PROVIDER: S-EPMC12650609 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature