Dual spatio-functional control of a fission yeast-based bioprocessor upon chemical induction.

ABSTRACT: Next-generation therapies are advancing beyond small molecules and proteins toward engineered living microorganisms that interact symbiotically with their host and respond to signals precisely when and where needed. Despite progress in the field, engineering cells to both produce biopharmaceuticals and achieve site-specific recruitment remains a challenge. In this work, we genetically engineered the mating pathway of S. pombe to create a "bioprocessor" that responds to a chemical trigger, an artificial replica of the sexual pheromone of the yeast cells, the P-factor, enabling functional control over the production of Albulin as a proof-of-concept biopharmaceutical. This activation simultaneously induces the expression of hydrophobic agglutinins on the cell surface, modifying surface

SUBMITTER: Sakellakou SM 

PROVIDER: S-EPMC12650765 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature