Establishment of Genetic Transformation System of Non-Embryogenic Callus in <i>Rosa rugosa</i>.

ABSTRACT: Rosa rugosa (R. rugosa) is a commercially important ornamental species within the genus Rosa, highly valued in the horticultural market. With the increasing availability and improved annotation of Rosa genomes, establishing an efficient genetic transformation system has become essential for validating candidate gene functions. As a common intermediate tissue in plant regeneration, callus has been successfully used to establish genetic transformation systems in numerous species. In this study, we characterized the morphological and physiological differences between embryogenic and non-embryogenic calli in R. rugosa. The embryogenic callus exhibited significantly higher catalase (CAT) activity and proline (PRO) content than the non-embryogenic callus. However, its growth

SUBMITTER: Liu X 

PROVIDER: S-EPMC12651251 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature