Development and Evaluation of Six Novel Recombinant GRA Proteins in Serodiagnosis of Human Toxoplasmosis.

ABSTRACT: Toxoplasma gondii is a globally distributed protozoan parasite, and reliable serodiagnosis is essential for effective management of toxoplasmosis. Conventional assays rely on tachyzoite lysate antigen (TLA), which suffers from limited standardization and reproducibility. In this study, immunodominant fragments of six dense granule proteins-GRA29, GRA35, GRA36, GRA45, GRA54, and GRA64-were expressed in Escherichia coli, purified, and evaluated as candidate antigens in IgG ELISAs using human sera. This study represents the first assessment of their diagnostic utility. Initial screening identified GRA29, GRA45, and GRA54 as promising candidates, with AUC values of 0.9983, 0.8507, and 0.9323, respectively, while GRA35-, GRA36-, and GRA64-based ELISA showed poor discrimination bet

SUBMITTER: Solowinska K 

PROVIDER: S-EPMC12651256 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature