Infection and resistance mechanisms of phage vB_VpM-PP270 in <i>Vibrio parahaemolyticus</i> via capsular polysaccharide.

ABSTRACT: Vibrio parahaemolyticus is a major foodborne pathogen that threatens public health and aquaculture, necessitating alternative biocontrol strategies such as phage therapy. This study introduces phage vB_VpM-PP270, isolated from aquaculture wastewater, and explores its infection mechanism targeting V. parahaemolyticus VP270. The research highlights capsular polysaccharide (CPS) as the primary receptor for phage adsorption and identifies ORF22, a tail spike protein, as the receptor-binding protein. PP270 demonstrates stability across a wide pH range and temperatures, effectively inhibiting VP270 growth in culture and on chilled shrimp. Phage-resistant mutants show mutations in CPS biosynthesis genes, which reduce phage adsorption and promote biofilm formation. These findings pro

SUBMITTER: Xihui Z 

PROVIDER: S-EPMC12651398 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature