Sp100A isoform promotes localization of the histone chaperone HIRA to PML nuclear bodies.

ABSTRACT: PML nuclear bodies (PML-NBs) are dynamic subnuclear structures important for chromatin dynamics and anti-viral defense. In this study we investigate the role of Sp100 isoforms in promoting localization of the H3.3 histone chaperone HIRA to PML-NBs in human keratinocytes. Sp100 knockout (KO) cell lines were generated in normal keratinocytes using CRISPR-Cas9 technology and shown to display typical skin development, normal PML-NB formation, and a normal interferon response as determined by RNAseq. However, the histone chaperone HIRA and its associated complex members (Ubinuclein-1 and ASF1A) failed to localize to PML-NBs in the absence of Sp100, even after interferon stimulation. Exogenous expression of the four main isoforms of Sp100 showed that the Sp100A isoform is the primary driver of H

SUBMITTER: Della Fera AN 

PROVIDER: S-EPMC12651510 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature