Whole Exome Sequencing for the Identification of Mutations in Bone Marrow CD34+Cells in Hodgkin Lymphoma.

ABSTRACT:

Background

Classical Hodgkin lymphoma (cHL) is a rare B-cell malignant neoplasm, characterized by the presence of rare mononucleated Hodgkin and multinucleated Reed-Sternberg cells (HRS). CD34+ cells are highly expressed on lymphoma stem cells in bone marrow (BM). Little is known about gene mutations in BM CD34+ cells of cHL. In this study, whole exome sequencing (WES) was performed and high-frequency mutation genes were examined through their expression levels.

Materials and methods

The influence of the variants on protein function was predicted with in silico tools or public databases. Gene expression levels were determined by quantitative real-time PCR.

Results

WES assay from BM CD34+ cells in thirty cHL patients revealed that three variants were detected in known

SUBMITTER: Trang PTH 

PROVIDER: S-EPMC12651673 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature