Screening and Identification of Reference Genes Under Different Conditions and Growth Stages of <i>Lyophyllum decastes</i>.

ABSTRACT: Internal reference genes are a prerequisite for ensuring the accuracy of gene verification experiments, but few relevant studies on Lyophyllum decastes have investigated the growth cycle and different environmental conditions. In this study, the qPCR results of 22 house-keeping genes were analyzed using GeNorm, BestKeeper, NormFinder and RefFinder. The results revealed that the most stable gene differed under different conditions. Across all developmental stages and under hot, cold, acidic, alkaline, and salt conditions, UBCE gene displays the greatest expression stability. However, EF1b, β-ACT, HSD17B3, and Cyb presented the greatest stability under cold, heat, and acidic conditions, and heavy metal exposure, respective

SUBMITTER: Hui YQ 

PROVIDER: S-EPMC12652064 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature