Integrated Enzyme-Mediated One-Step Sample Processing and Duplex Amplification System for Rapid Detection of <i>Carpione rhabdovirus</i> in Aquaculture-Derived Food Products.

ABSTRACT: Golden pompano (Trachinotus ovatus) is the largest-scale marine aquaculture fish species in China, with a significant economic and nutritional value as a high-quality seafood product. The recent outbreak of an epidemic caused by a novel Carpione rhabdovirus (CAPRV) occurred in cultured golden pompano. To address it, a CAPRV enzyme-mediated one-step sample processing-reverse transcription-enzyme-mediated duplex exponential amplification (EmOSP-RT-EmDEA) detection system was developed. This innovative molecular diagnostic tool integrates enzyme-mediated one-step sample processing (EmOSP) with enzyme-mediated duplex exponential amplification (EmDEA) technology. Unlike traditional RPA-Cas12a detection methods, this system directly incorporates fluorophores into RNA components, el

SUBMITTER: Sun H 

PROVIDER: S-EPMC12652080 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature