Molecular Characterization and Expression Patterns of <i>Sox3</i> and <i>Sox30</i> Genes and Response to Exogenous Hormones in the Chinese Soft-Shelled Turtle (<i>Pelodiscus sinensis</i>).

ABSTRACT:

Background/objectives

The Sox transcription factor family is critical for gonadal development and sex differentiation in animals, yet its roles in chelonians, particularly in the Chinese soft-shelled turtle (Pelodiscus sinensis), have rarely been investigated.

Methods

This study cloned and analyzed the cDNA sequences of Sox3 and Sox30 genes from P. sinensis, examining their amino acid sequences and structural properties. Real-time quantitative PCR (RT-qPCR) was used to assess the expression of these two genes in different adult tissues and at various stages of embryonic gonadal development. Additionally, the effects of exogenous hormones (17β-estradiol, E₂ and 17α-Methyltestosterone, MT) on the expression of Sox3 and Sox30

SUBMITTER: Xiao K 

PROVIDER: S-EPMC12652197 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature