DNA Barcoding Identification of <i>Angelicae Sinensis</i> Radix and Its Adulterants Based on Internal Transcribed Spacer 2 Region and Secondary Structure Prediction.

ABSTRACT: Background: Morphological similarities among Angelicae Sinensis Radix and related species often lead to market substitution. This study develops a DNA barcoding method to authenticate these herbs and identify adulterants derived from Angelica, Heracleum, and Peucedanum genera. Methods: Phylogenetic analysis was conducted using MEGA 11.0 software with ITS2 sequences from Angelicae Sinensis Radix, Ligusticopsis Pubescens Radix, Angelicae Pubescens Radix, and their related species within the genera Angelica, Peucedanum, and Heracleum. Additionally, ITS2 secondary structures were predicted for the three herbs to provide supplementary evidence for identification. Results: The amplification success rate wa

SUBMITTER: Chen Z 

PROVIDER: S-EPMC12652221 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature