TOP2B modulates DNA supercoiling and chromatin contacts during transcriptional induction.

ABSTRACT: Human type-II topoisomerases, TOP2A and TOP2B, resolve transcription-associated DNA supercoiling, thereby influencing gene expression programs, and have been recently linked to three-dimensional genome architecture through yet poorly understood mechanisms. Here, we investigate the regulatory roles of TOP2 paralogs using estrogen signaling, which triggers an acute transcriptional induction that involves extensive rewiring of genome organization, as a model system. Unexpectedly, we find that estrogen treatment strongly inhibits TOP2B catalytic activity-although not its binding-specifically at estrogen-responsive enhancers and promoters. This inhibition results in an accumulation of negative DNA supercoiling and promotes the formation of regulatory chromatin contacts. Estrogen-mediated inhibi

SUBMITTER: Terron-Bautista J 

PROVIDER: S-EPMC12652261 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature