STR-Uggles: Overcoming Humic Acid Inhibition Using Combined STR & qPCR Kit Chemistries.

ABSTRACT:

Background/objectives

DNA profiling can fail, or produce poor results, when naturally occurring materials are present during the amplification step. This study demonstrates that simple modifications to the reaction setup can overcome this obstacle. PCR inhibition is caused by a range of compounds including haem, humic acid and dyes. Various strategies to overcome this inhibitory effect have been explored, such as improving extraction methods to remove these compounds, diluting samples to reduce inhibitor concentration, or using inhibitor-tolerant DNA polymerases. In this study, we evaluate whether modified setups can help mitigate the effects of humic acid, a common inhibitor that induces various inhibition mechanisms.

Methods

We combined the GlobalFiler STR kit with Investi

SUBMITTER: McDonald C 

PROVIDER: S-EPMC12652367 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature