Mapping-by-Sequencing via eBSRmap (Easy Bulk Segregate RNA Mapping) in a B73 EMS Mutant Population.

ABSTRACT:

Background

Maize, crucial for food, feed, and industry, is a model for genetic and breeding research. Kernel traits directly affect maize yield. This study developed the eBSRmap method to simplify gene cloning related to maize kernel traits.

Methods

The eBSRmap method constructs a maize EMS mutant population, then conducts RNA-seq on pooled mutant and wild-type samples to identify SNP markers and map candidate genes for kernel trait mutations.

Results

Applied to a maize EMS mutant population, eBSRmap identified candidate genes for twenty kernel trait mutants, successfully mapped twelve, and eight were confirmed by co-segregation analysis (success rate: 40%). The identified genes showed mutations like missense and stop-gained, related to phenotypes such as small, shrun

SUBMITTER: Cui Y 

PROVIDER: S-EPMC12652423 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature