CircRNA-02213 Regulates Milk Fat Synthesis in Bovine Mammary Epithelial Cells via ACSS2.

ABSTRACT:

Background

In the bovine mammary gland, de novo fatty acid synthesis is a critical process for milk fat production, in which acetyl-CoA synthetase 2 (ACSS2) serves as a key enzyme by converting acetate into acetyl-CoA. This metabolic pathway is intricately regulated by non-coding RNAs, particularly through the competitive endogenous RNA (ceRNA) mechanism.

Purpose

To elucidate the regulatory role and molecular mechanism of the circRNA-02213/miR-328/ACSS2 axis in the lipid metabolism of bovine mammary epithelial cells (BMECs).

Methods

Bioinformatic prediction and dual-luciferase reporter assays were employed to verify the targeting interactions among circRNA-02213, miR-328, and ACSS2. In BMECs, qRT-PCR, Western blot, triglyceride/cholesterol quantification, Oil Red O st

SUBMITTER: Sui M 

PROVIDER: S-EPMC12652434 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature