Impact of Charge Reduction on Sequence Coverage of Proteins by 193 nm Ultraviolet Photodissociation.

ABSTRACT: An ongoing goal of top-down mass spectrometry is to increase the performance for larger proteins. Using higher energy activation methods, like 193 nm ultraviolet photodissociation (UVPD), offers the potential to cause more extensive fragmentation of large proteins and thereby yield greater sequence coverage. Obtaining high sequence coverage requires confident identification and assignment of fragment ions, and this process is hampered by spectral congestion and low signal-to-noise ratio (S/N) of the fragment ions. Here we explore the use of charge reduction methods to produce lower charge states of large proteins to increase ion accumulation and generate lower charge states of fragment ions, ones that are naturally dispersed in the m/z domain to alleviate spectral congestion.

SUBMITTER: Dunham SD 

PROVIDER: S-EPMC12652453 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature