Identification of Male-Specific Markers by Genotyping-by-Sequencing in the Giant Spiny Frog, <i>Quasipaa spinosa</i>.

ABSTRACT:

Background/objectives

Quasipaa spinosa, a large-sized spiny frog, has high commercial value in the food trade. Although the sexual dimorphism in body size between males and females has been investigated, the sex-determining mechanism in this frog remains unknown.

Methods

This study employed a genotyping-by-sequencing (GBS) method to identify sex-associated genomic markers and elucidate the sex determination mechanism in the species.

Results

We obtained 853 candidate sex-specific GBS tags, with 811 tags (95.07%) demonstrating a male heterozygous system (XX/XY). The diagnostic specificity of the nine markers was further demonstrated by PCR analysis across multiple adult individuals from seven distinct geographic populations of the frog. Four sex-specific markers

SUBMITTER: Xiao Y 

PROVIDER: S-EPMC12652585 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature