Structural Conservation and Transcriptional Plasticity of <i>atp2a1</i> in <i>Acrossocheilus fasciatus</i> Under Temperature and Flow Acclimation.

ABSTRACT:

Background/objectives

The sarcoplasmic reticulum Ca²⁺-ATPase 1 (Atp2a1) is a key regulator of calcium homeostasis and muscle relaxation, yet its roles in fish remain poorly understood.

Methods

We investigated the structural characteristics, phylogenetic relationships, and transcriptional regulation of atp2a1 in Acrossocheilus fasciatus, a stream-dwelling cyprinid sensitive to environmental fluctuations.

Results

Bioinformatic analyses revealed that the 991-aa Atp2a1 protein is highly conserved among teleosts but exhibits divergence from mammals in the Cation_ATPase_N domain and transmembrane regions TM3, TM9, and TM10. Phylogenetic analysis clustered A. fasciatus most closely with Onychostoma macrolepis. Tissue-specific qRT-PCR de

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12652649 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature