Semaphorin3A Rewires CD4⁺ T-Cell Metabolism via AKT/mTORC1 Inhibition in Health and Rheumatoid Arthritis.

ABSTRACT: Semaphorin3A (Sema3A) is a regulatory protein found to be expressed on regulatory T and B cells and also secreted into peripheral blood. It has been identified as a potent immune regulator; however, not all its regulatory mechanisms have been evaluated. In this respect, we aim to investigate how Sema3A affects key metabolic pathways in T cells during homeostasis and rheumatoid arthritis (RA), and on the AKT/mTORC1 signaling axis. In this study, peripheral blood samples were collected from 119 healthy donors and 32 rheumatoid arthritis patients. T cells were subjected to Seahorse analysis to evaluate OXPHOS and glycolysis, live cell TMRE staining to evaluate mitochondrial activity, mass spectrometry for metabolite profiling, ATP determination to study ATP production, and Western blot analys

SUBMITTER: Mubariki R 

PROVIDER: S-EPMC12652675 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature