<i>Lactobacillus rhamnosus</i> GG and <i>Lactobacillus paracasei</i> IMPC2.1 Mitigate LPS-Induced Epithelial Barrier Dysfunction: A Focus on Autophagy Regulation.

ABSTRACT: The intestinal epithelial barrier is critical for maintaining gut homeostasis, yet its integrity can be compromised by inflammation and microbial dysbiosis. Here, we demonstrate that Lactobacillus rhamnosus GG (LGG) and Lactobacillus paracasei IMPC2.1 (L. paracasei) show their effectiveness in enhancing epithelial barrier function and modulating autophagy, counteract the epithelial barrier dysfunction, induced by Lipopolysaccharide (LPS), in Caco-2 cells by modulating tight junction (TJ) protein expression through regulation of inflammation and apoptosis. LPS exposure significantly reduced transepithelial electrical resistance (TEER) and increased paracellular permeability, effects that were partially reversed by both probiotic strains. Western blot analysis revealed t

SUBMITTER: Orlando A 

PROVIDER: S-EPMC12652688 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature