Elucidating the Chemistry Behind Thiol-Clickable GelAGE Hydrogels for 3D Culture Applications.

ABSTRACT: Although covalently crosslinked gelatin hydrogels have been investigated for use in 3D cell culture due to inherent bioactivity and proliferation within the denatured collagen precursor, the stability of the matrix, and relatively inexpensive synthesis, current systems lack precise control over mechanical properties, including homogeneity, stiffness, and efficient diffusion of nutrients to embedded cells. Difficulties in modifying gel matrix composition and functionalization have limited the use of covalently crosslinked gelatin hydrogels as a three-dimensional (3D) cell culture medium, lacking the ability to tailor the microenvironment for specific cell types. In addition, the currently utilized chain-growth photopolymerization mechanism for crosslinking hydrogels has a potential for side

SUBMITTER: Swank S 

PROVIDER: S-EPMC12652731 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature