Distinct Morphokinetic Signature of Human Embryos with Chromosomal Mosaicism.

ABSTRACT:

Objectives

This study aimed to determine whether chromosomal mosaicism in blastocysts is associated with a distinct morphokinetic signature.

Methods

Preimplantation genetic testing for aneuploidy (PGT-A) was performed on 182 human embryos via trophectoderm biopsy on day 5 and analyzed by next-generation sequencing. Embryos were classified as euploid (n = 55), mosaic (n = 39: 21 low-grade, 18 high-grade), or aneuploid (n = 88), of which 18 with concurrent mosaicism. Prior to biopsy, embryos were cultured in a time-lapse system (EmbryoScope), and 12 morphokinetic parameters were assessed, including pronuclei fading (tPNf), cleavage times (t2-t9), morula formation (tM), blastulation start (tSB), and full blastocyst formation (tB). These parameters were compared according to plo

SUBMITTER: Ruseva M 

PROVIDER: S-EPMC12652751 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature