Cross-Species Validation of Pigeon-Specific CHD1 Primers for Molecular Sexing in Pet Birds.

ABSTRACT: Young and adult birds of a large number of species are sexually monomorphic. The development of molecular methodologies for sexing birds has overcome these difficulties, allowing reliable sex differentiation. An important gene in sex determination across a variety of bird species is the CHD1 gene, which encodes Chromodomain-helicase DNA binding protein-1 and is located on the avian Z and W chromosomes. The aim of the study is to evaluate the cross-species performance of pigeon-specific CHD1 primers in identifying the molecular sex of birds from five different families, alongside the universal CHD1F/CHD1R primers. The samples were collected from birds of different ages from five different families (Psittaculidae, Psittacidae, Columbidae, Fringillidae, and

SUBMITTER: Marc S 

PROVIDER: S-EPMC12652837 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature