Production and Optimization of Anti-Aging Peptides from <i>Pleurotus eryngii</i> Mushroom Feet: Mechanistic Insights via Integrated Transcriptomics and Metabolomics.

ABSTRACT: This study established an optimized process for obtaining anti-aging peptides from Pleurotus eryngii mushroom feet (PEMFPeps). Using response surface methodology, high yields of protein (51.31 ± 3.00%) and peptides (48.71 ± 0.17% hydrolysis degree) were achieved. In a D-galactose-induced PC12 cell aging model, the simulated digests (SID-PEMFPeps) exhibited potent anti-aging effects at a concentration of 1 mg/mL. An integrated transcriptomic and metabolomic approach was employed to systematically investigate the underlying mechanisms. The results revealed that Integrated transcriptomic and metabolomic analyses showed that SID-PEMFPeps alleviated cellular senescence through multi-dimensional regulation of transcriptional and metabolic networks. This included modulating key pathways re

SUBMITTER: Wang S 

PROVIDER: S-EPMC12652877 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature