Untargeted Metabolomics Identifies Faecal Filtrate-Derived Metabolites That Disrupt <i>Clostridioides difficile</i> Metabolism and Confer Gut Barrier Cytoprotection.

ABSTRACT: Recurrent Clostridioides difficile infection (rCDI) remains a major therapeutic challenge. Although faecal microbiota transplantation (FMT) is highly effective and thought to restore microbial composition and metabolic function, the mechanisms underlying its success are not fully understood. In particular, the contribution of non-bacterial components such as soluble metabolites remains unclear. Therefore, further investigation is needed to identify the mechanistic drivers of FMT efficacy and clarify how non-bacterial factors contribute to therapeutic outcomes. Here, we applied untargeted three-dimensional Orbitrap secondary ion mass spectrometry (3D OrbiSIMS) to profile faecal metabolic reprogramming in rCDI patients pre- and post-FMT, alongside C. difficile cultures exposed

SUBMITTER: Qassadi FI 

PROVIDER: S-EPMC12653063 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature