Identifying a Marine-Derived Small-Molecule Nucleoprotein Inhibitor Against Influenza A Virus.

ABSTRACT: Influenza A virus (IAV) poses a major threat to global public health, exerting immense pressure on human health and the economy. The IAV nucleoprotein (NP) is an ideal target for antiviral drug development. Through Mini-genome and Surface Plasmon Resonance assays, this study discovered and verified that mycophenolic acid methyl ester (MAE), a secondary metabolite produced by the marine algal-associated fungus Phaeosphaeria spartinae, can target the viral nucleoprotein to exert anti-IAV activity. Pull-down assays and immunofluorescence have revealed that MAE blocks the nuclear import of viral ribonucleoprotein complexes (vRNP) by interfering with the interaction between NP and IMP-α. It also affects the vRNP assembly process by regulating NP oligomerization and the interaction betwee

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12653688 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature