Endotoxin Pretreatment Mitigates Myocardial Ischemia-Reperfusion Injury Through Preservation of Mitochondrial Respiration: A Combined Assessment of In Vivo, Ex Vivo, and In Vitro Data.

ABSTRACT: Acute myocardial infarction is the most common form of coronary artery disease, and myocardial ischemia-reperfusion injury remains a major challenge despite advances in reperfusion therapy. Endotoxin preconditioning has been linked to reduced ischemia-reperfusion injury, but mechanisms remain unclear, and prior studies have used varied assessment methods with inconsistent results. In this study, we confirmed the protective effect of endotoxin preconditioning and assessed its role in preserving mitochondrial respiration using a multi-model approach of in vivo ischemia-reperfusion rat model, ex vivo normothermic rat heart perfusion, and in vitro hypoxia-reoxygenation in neonatal rat cardiomyocytes. Hemodynamic and cell-based analyses were performed in control (n = 5), ischemia-reperfu

SUBMITTER: Habimana R 

PROVIDER: S-EPMC12653926 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature