PCB 37 (3,4, 4'-trichlorobiphenyl) increased apoptosis and modulated neuronal morphogenesis in primary rat cortical neuron-glia cocultures in a concentration-, sex-, age-, and CREB-dependent manner.

ABSTRACT: Higher-chlorinated (HC) polychlorinated biphenyls (PCBs) are known developmental neurotoxicants. In contrast, there are limited data regarding the developmental neurotoxicity of lower-chlorinated (LC) PCBs despite the increasing environmental prevalence and detection of LC-PCBs in contemporary human tissues, including the perinatal brain. This study characterized the neurotoxic effects of the LC-PCB congener PCB 37 in primary male and female rat cortical neuron-glia co-cultures. Cultures were exposed to varying concentrations of PCB 37 for 48 h beginning on day in vitro 0 or 7. Cell viability was assessed by measuring lactate dehydrogenase release into the culture medium and the percentage of live cells identified using Calcein-AM and Hoechst staining. Apoptosis was measured using fluorome

SUBMITTER: Badley JR 

PROVIDER: S-EPMC12653993 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature